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In molecular biology, TBST (or TTBS) is a mixture of tris-buffered saline (TBS) and Polysorbate 20 (also known as Tween 20). It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.
Video TBST
Contents of TBS-Tween
The following is a sample recipe for TBST:
- 50 mM Tris
- 150 mM NaCl
- 0.1% Tween 20
Adjust pH with HCl to pH 7.4-7.6
The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.
Maps TBST
References
http://www.seattlechildrens.org/pdf/wholemount-IHC-protocol-1.pdf
Source of article : Wikipedia
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